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Pgadt7 Ad. Use text editor or plasmid mapping software to view sequence. Test the y1hgold bait strain for background aba r.
Smai can be used at 37°c for brief incubations. Hominis, cp265, cp266 or cp267 from c.
Snapgene Viewer Is Free Software That Allows Molecular Biologists To Create, Browse, And Share Richly Annotated Sequence Files.
Close the tube and incubate for 10 minutes at room temperature.
Smai Can Be Used At 37°C For Brief Incubations.
Nde i, sfi i, eco.
Pgadt7 Ad Is A Yeast Expression Vector That Is Designed To Express A Fusion Protein Between The Gal4 Activation Domain (Ad;
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Snapgene Viewer Is Free Software That Allows Molecular Biologists To Create, Browse, And Share Richly Annotated Sequence Files.
Carefully open the tube and add 20 μl of sterile water to dissolve the dna.
Pgadt7 Ad Includes The T7 Promoter Between The Gal4 Ad And The Epitope Tag.
Clontech (takara) open in snapgene.
This Recognition Sequence Is Asymmetric, So Ligating Blunt Ends.